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| 1. |  | FARIA, C. U. de; MAGNABOSCO, C. de U.; ALBUQUERQUE, L. G. de; REYES, A. de los; PIRES, B. C.; LEMOS, M. V. A. de; LÔBO, R. B. Análise genética de escores visuais em bovinos da raça nelore, na desmama até a maturidade. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 46., 2009, Maringá. Inovação científica e tecnológica em zootecnia: anais dos resumos. Maringá: SBZ: UEM, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 2. | | PERIPOLLI, E.; OLIVIERI. B. F.; FEITOSA, F. L. B.; LEMOS, M. V. A. de; TONUSSI, R. L.; MUNARI, D. P.; PANETTO, J. C. do C.; MACHADO, M. A.; VENTURA, R. V.; BALDI, F.; SILVA, M. V. G. B. Avaliação dos coeficientes de endogamia em bovinos da raça Gir (Bos primigenius indicus) In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto. Anais... Ribeirão Preto: SBMA, 2017. 3 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | PERIPOLLI, E.; METZGER, J.; LEMOS, M. V. A. de; STAFUZZA, N. B.; KLUSKA, S.; OLIVIERI, B. F.; FEITOSA, F. L. B.; BERTON, M. P.; LOPES, F. B.; MUNARI, D. P.; LOBO, R. B.; MAGNABOSCO, C. de U.; DI CROCE, F.; OSTERSTOCK, J.; DENISE, S.; PEREIRA, A. S. C.; BALDI, F. Autozygosity islands and ROH patterns in Nellore lineages: evidence of selection for functionally important traits. BMC Genomics, v. 19, 680, 2018.| Biblioteca(s): Embrapa Cerrados. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/05/2017 |
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Data da última atualização: |
22/11/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, J. M. L. dos; MONTEIRO, J. P.; RIBEIRO, W. L. C.; MACEDO, I. T. F.; ARAÚJO FILHO, J. V. de; ANDRE, W. P. P. |
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Afiliação: |
Jessica Maria Leite dos Santos, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; Wesley Lyeverton Correia Ribeiro, UECE - Fortaleza, CE, Brazil; Iara Tersia Freitas Macedo, UECE- Fortaleza, CE, Brazil; José Vilemar de Araújo Filho, UECE- Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECE - Fortaleza, CE, Brazil. |
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Título: |
High levels of benzimidazole resistance and beta-tubulin isotype 1 SNP F167Y in Haemonchus contortus populations from Ceará State, Brazil. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Small Ruminant Research, Amsterdam v. 146, p. 48-52, Jan. 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infected with 5000 L3, and nematode eggs were collected for molecular test before and after anthelmintic treatments. The results showed preferential selection of SNP F167Y in response to oxfendazole, an increase in resistant SNP frequencies in general in response to albendazole and little change in relation to pre-treatment situations in response to ivermectin. Our results confirm that BZ resistance is common. The resistant allele at SNP F167Y in H. contortus prevails in Ceará State, and we provide evidence that this result may be due to the utilization of oxfendazole in recent years. MenosAbstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infect... Mostrar Tudo |
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Palavras-Chave: |
B-tubulin; Brasil; Ceará; Nematóide gastrointestinal; Real-time PCR; Resistance to anthelmintics; SNP. |
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Thesagro: |
Anti-helmíntico; Benzimidazol; Haemonchus Contortus; Helminto gastrintestinal; Ovino; Resistência. |
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Thesaurus NAL: |
Benzimidazoles; Brazil; Nematoda; Sheep. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03219naa a2200385 a 4500
001 2069622
005 2018-11-22
008 2017 bl uuuu u00u1 u #d
100 1 $aSANTOS, J. M. L. dos
245 $aHigh levels of benzimidazole resistance and beta-tubulin isotype 1 SNP F167Y in Haemonchus contortus populations from Ceará State, Brazil.$h[electronic resource]
260 $c2017
520 $aAbstract: Haemonchus contortus is the most prevalent parasitic nematode in tropical areas, and anthelmintic resistance is a global problem. Our objective was to characterize benzimidazole (BZ) resistance in gastrointestinal nematode populations in Ceará State, Brazil, using the egg hatch test (EHT) and in H. contortus populations using quantitative real-time polymerase chain reaction (qPCR). Twenty locations were surveyed, and fecal samples were collected from a minimum of 40 animals from each farm and pooled. Five thousand L3 from each farm were used to infect single animals at Embrapa (Brazilian Agricultural Research Company) to provide a source of eggs for both phenotypical and molecular tests. The mean EHT was 2.46 ug/ml (±0.58 g/ml), and BZ resistance was detected at all surveyed locations. The mean resistant allelic frequencies at positions F200Y and F167Y were 34.16% (±12.13%) and 58.31% (±18.89%), respectively. The resistant allelic frequencies at F167Y were higher than those at F200Y in most studied locations. We also investigated the possibility that specific BZ utilization may influence resistant allelic frequencies. We selected three nematode populations based on the resistant SNP prevalence at F200Y and F167Y as follows: higher frequency at SNP F200Y, higher frequency at SNP F167Y and similar frequencies at both positions. Anthelmintic treatments included two BZs (oxfendazole and albendazole) and ivermectin. Three animals per population per treatment were infected with 5000 L3, and nematode eggs were collected for molecular test before and after anthelmintic treatments. The results showed preferential selection of SNP F167Y in response to oxfendazole, an increase in resistant SNP frequencies in general in response to albendazole and little change in relation to pre-treatment situations in response to ivermectin. Our results confirm that BZ resistance is common. The resistant allele at SNP F167Y in H. contortus prevails in Ceará State, and we provide evidence that this result may be due to the utilization of oxfendazole in recent years.
650 $aBenzimidazoles
650 $aBrazil
650 $aNematoda
650 $aSheep
650 $aAnti-helmíntico
650 $aBenzimidazol
650 $aHaemonchus Contortus
650 $aHelminto gastrintestinal
650 $aOvino
650 $aResistência
653 $aB-tubulin
653 $aBrasil
653 $aCeará
653 $aNematóide gastrointestinal
653 $aReal-time PCR
653 $aResistance to anthelmintics
653 $aSNP
700 1 $aMONTEIRO, J. P.
700 1 $aRIBEIRO, W. L. C.
700 1 $aMACEDO, I. T. F.
700 1 $aARAÚJO FILHO, J. V. de
700 1 $aANDRE, W. P. P.
773 $tSmall Ruminant Research, Amsterdam$gv. 146, p. 48-52, Jan. 2017.
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